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#dotblot #RohitMane #ScientistRacademy Dot Blot is a cheaper, easier and faster technique to detect the presence of Proteins and Nucleic Acids in a biological sample. Due to the simplicity of the technique it widely used as a ideal diagnostic tool. This post covers the Protein Dot Blot Technique. Dot Blot Principle Dot Blot works based on the immunodetection principle for identifying specific protein. Dot Blot - PrincipleDot Blot - Technique: Overview Proteins are immobilized on to a protein binding membrane, PVDF or Nitrocellulose. After Protein Immobilization membrane is blocked to avoid non-specific binding, using blocking buffer. Immobilized protein is then incubated with a primary antibody (for 30mins to 1 hour). The antibody is specific to the protein and is visualized either with a specific tag or with a secondary antibody.  The secondary antibody generally will carry a tag that allow the visualization of protein. Blots can be visualized using methods such as Chemiluminescence or Fluorescence. Common Tags used in Dot Blots are enzymes conjugated to the secondary antibody. Upon addition of substrate, enzyme acts on the substrate to develop colour. Commonly preferred enzymes are HRP(Horse Radish Peroxidase) and Alkaline Phosphatase (AP) Advantages of Dot Blot over Western Blot Non-Electrophoresis Technique: Dot Blot is non-electrophoresis technique, means that one need not do protein electrophoresis. Proteins can directly immobilized on to the membrane. Simple & Easy Method: No messy work of PAGE run and gel to membrane transfers, proteins are directly immobilized on membrane, making this method much easier and faster. Time: It is a faster method, takes ~3hours, whereas western blot may even take up to 2 days. Including the protein electrophoresis and gel to membrane transfer optimization. Dot Blot is not as informative as Western Blot, but it is inexpesive, quick and easy method for the simple protein detection.  dot blot technique by shomus biology dot blot technique in hindi

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Subscribe for more videos and tips: https://www.youtube.com/channel/UC_3WB1wfJaItIiN2RJsRMOw?sub_confirmation=1 The Tropicana Casino, located on the famed Atlantic City Boardwalk, opened its doors in 1981 and quickly became one of the city's most iconic casino resorts. Originally developed on the site of the former Ambassador Hotel, the Tropicana was envisioned as a sprawling entertainment destination that combined classic Atlantic City glamour with the modern flair of Las Vegas. Over the decades, the property underwent several major renovations and ownership changes, expanding to include the Havana Tower, The Quarter shopping and dining complex, and one of the largest hotel accommodations in New Jersey. Known for its vibrant atmosphere, tropical theme, and 24/7 energy, the Tropicana played a key role in revitalizing Atlantic City’s casino scene during its peak years and remains a symbol of the city’s rollercoaster fortunes in gaming and tourism.

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This FlowRider is located on the 6th floor pool deck at Planet Hollywood Resort on the Las Vegas strip. It is open to the public as well as guest. The price is 35$ for 30 minutes or $25 with a local ID. There is a trainer as well as stand up flowboards or bodyboards. Unique Surfing Experience in the Desert: It offers the thrill of catching a wave in the middle of the desert, which is a novel and exciting concept, especially for teens who might not have access to real ocean surfing. This makes it a memorable and unique experience. Action-Packed and Engaging: Unlike simply lounging by a pool, the FlowRider provides an active and challenging experience. Teens can try bodyboarding or standing up to surf, practicing their balance and skills. This active engagement is often more appealing to teenagers than passive relaxation. Suitable for Various Skill Levels: Whether they are complete beginners or have some boarding experience, the FlowRider is designed to accommodate different skill levels. Instructors are on hand to guide newbies, and the special impact-absorbing surface minimizes the fear of wiping out, making it approachable. More experienced riders can still enjoy the challenge and try tricks. Cool Way to Beat the Las Vegas Heat: Summer in Las Vegas can be extremely hot. The FlowRider provides an exhilarating and effective way to cool down and have fun in the water, offering a refreshing escape from the intense temperatures. Social and Interactive: The FlowRider is often part of a lively pool deck scene. Teens can go with friends, watch each other ride, and share in the excitement, making it a social and interactive activity. There's an element of friendly competition and encouragement. Convenient Location: Located on the 6th-floor rooftop pool deck of Planet Hollywood, it's easily accessible on the Las Vegas Strip, making it a convenient option for teens staying at or near the hotel. "Cool Factor": Surfing, even simulated, carries a certain "cool factor" that appeals to teenagers. It's an opportunity to try something a bit more extreme and show off their adventurous side. While it's important to note that there are age and height requirements (42 inches for bodyboarding and 48 inches for standing up), the FlowRider at Planet Hollywood provides an active, thrilling, and unique water experience that makes it a top choice for teens looking for summer fun in Las Vegas. This is a great Las Vegas attraction for kids, teens and adults ! Looking for a family-friendly activity in Las Vegas!? Look no further, this action-packed/extreme sport surfing machine is the best way to beat the heat on the Las Vegas Strip. Leave a comment and Subscribe to help grow the sport of flowboarding around the world. instagram.com/flowrider13roz www.facebook.com/Flowrider13r0z www.13roz.com Surf this wave in the city of Las Vegas. Tags FlowRider, Flowboarders, FlowHouse, Wave House, Flowboarding, Flowboard, Surfrider, Flowriding, flowride, flow boarding, surf machine, surf pool, waveloch, whitewater, whitewaterwest, surfing, bodyboarding, bodyboard, bodyboarder, surfer, skatepark, skateboarding, skate white water west, flowboarders, Flowrider, flowboarding, flow rider, flow riding, flowride, flowboard, flowriding, flow tour, floriding, floride, flowride, flowride, contest, competition, worlds best tricks and fails, maeva surf, aqua shop, wave house, wavehouse, flow house, flowboards, salomon center, Royal Caribbean, flowhouse bangkok, surf house phuket, iaapa, water park thrill rides, surfing machine, wave machine, surf simulator, Wave pool, surfboarding, sheet wave, adg, aquatic development group, pacific surf designs, psd, sean silveira, nick nguyen, daniel tarapchak, scott callens, city wave, waikai lineup, las vegas fun

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First day in Vegas! From Buddy V's Ristorante to California's Market Street Cafe, today was full of good eats! Check out our Instagram for more pics from our adventures! lewhoo220 and tacobill35. Also check out our Facebook page under Bill & Lisa's Travel Page for a preview on where we are before the vlog comes out. Thanks so much for watching! Buddy V’s Ristorante, 3327 S Las Vegas Blvd, The Venetian, Las Vegas, NV 89109 Market Street Cafe, 12 E Ogden Ave, Las Vegas, NV 89101

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